A packed column supercritical fluid chromatography (SFC) method for the
separation of clopidogrel enantiomers on a chiral stationary phase and CO2 with modifier as mobile phase has been developed at an analytical scale. Among
11 different 2 stationary phases the Chiral cel OD-H column showed by far the
best separation properties. The influence of different modifiers, injection
solvents, temperature, and pressure, and density of the fluid, respectively, on
the separation behaviour
has been studied. It was found that the separation behaviour strongly depends
on the type of modifier and the modifier content. Temperature and pressure are
of less influence.
P. W. Majerus and D. M. Tollefsen, “Blood Coagulation and Anticoagulant, Thrombolytic, and Antiplatelet Drugs,” In: L. L. Brunton, Ed., The Pharmacological Basis of Therapeutics, 11th Edition, The McGraw-Hill Companies, New York, 2006, p. 1483.
J. M. Herbert, D. Frehel, E. Vallee, G. Kieffer, D. Gouy, Y. Berger, G. Defreyn and J. P. Maffrand, “Clopidogrel, a Novel Antiplatelet and Antithrombotic Agent,” Cardio- vas-cular Drug Reviews, Vol. 11, No. 2, 1993, pp. 180-198.
P. Lagorce, Y. Perez, J. Ortiz, J. Necciari and F. Bressolle, “Assay Method for the Carboxylic Acid Metabolite of Clopidogrel in Human Plasma by Gas Chromatography- Mass Spectrometry,” Journal of Chromatography B: Bio-medical Sciences and Applications, Vol. 720, No. 1-2, 1998, pp. 107-117. doi:10.1016/S0378-4347(98)00452-6
H. Ksycinska, P. Rudzki and M. Bukowska-Kiliszek, “De- termination of Clopi-dogrel Metabolite (SR26334) in Human Plasma by LC-MS,” Journal of Pharmaceutical and Biomedical Analysis, Vol. 41, No. 2, 2006, pp. 533-539.
A. Mitakos and I. Panderi, “Determination of the Carbox-ylic Acid Metabolite of Clopi-dogrel Inhuman Plasma by Liquid Chromatography-Electrospray Ionization Mass Spectrometry,” Analytica Chimica Acta, Vol. 505, No. 1, 2004, pp. 107-114. doi:10.1016/S0003-2670(03)00019-9
S. S. Singh, K. Sharma, D. Barot, P. R. Mohan and V. B. Lohray, “Estimation of Carboxylic Acid Metabolite of Clopidogrel in Wistar Rat Plasma by HPLC and Its Application to a Pharmacokinetic Study,” Journal of Chro- matography B, Vol. 821, No. 2, 2005, pp. 173-180.
E. Souri, H. Jalali-zadeh, A. Kebriaee-Zadeh, M. Shekarchi and A. Dalvandi, “Validated HPLC Method for Determination of Carboxylic Acid Metabolite of Clopidogrel in Human Plasma and Its Application to a Pharmacoki-netic Study,” Biomedical Chromatography, Vol. 20, No. 12, 2006, pp. 1309-1314. doi:10.1002/bmc.697