The antibacterial activity of honey is well documented, this activity is mainly due to its low pH, osmolarity and hydrogen peroxide accumulation. Recently, more attention has been given to the importance of a unique extra antimicrobial activity, termed as a non-peroxide activity. The aim of this work was to investigate the antimicrobial activity of selected honeys from different origins; specifically to evaluate their non-hydrogen peroxide derived activity, against Staphylococcus epidermidis, Bacillus sphaericus, Bacillus subtilis, Serratia marcescens, Escherichia coli and S. epidermidis; manuka honey was used as the control. Antibacterial activity of the honeys was assayed using standard well diffusion methods. noticeable variations in the antibacterial activity of the different honey samples were observed. Most of tested honeys had broad-spectrum antibacterial activity, particularly Greek Pine, Scottish Heather, Chilean Ulmo, New Zealand Beech and Jarrah Honey. Unfortunately, none of the tested honey had a detectable non-peroxide activity.