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Genetic diversity among Puntius sophore complex using restriction fragment length polymorphism

Keywords: RFLP , Hind III , genetic diversity , Puntius sophore complex

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Abstract:

Restriction fragment length polymorphism (RFLP) analysis of genomic DNA was employed to characterize species of Puntius sophore complex, a benthopelagic freshwater fish species. Samples of five species of Puntius viz, Puntius sophore, Puntius amphibius, Puntius chola, Puntius bimaculatus and Puntius dorsalis from the East-flowing Tamiraparani and Kuzhithurai and West-flowing Tamiraparani river basins (Western Ghats) were analyzed. The restriction enzyme employed (Hind III) generated species-specific DNA patterns for each of the five species. DNA exhibited considerable polymorphism among species. All samples showed relatively high values of diversity. The relation between species is discussed.

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