Imaging by means of Raman spectroscopy has emerged as a powerful technique in the study of various chemical processes occurring in biology. This technique is non-invasive, label-free, capable of providing molecular identity and can be performed in robust conditions. However, one major drawback is its inherently weak signal. The ways to overcome this issue is to use Raman based methods e.g. Resonance Raman Spectroscopy (RRS), Surface Enhanced Raman Spectroscopy (SERS), Tip Enhanced Raman Spectroscopy (TERS). In this review, we gave a brief introduction of all these methods, with special emphasis on their recent advances and applications in various fields of life science.