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Marker Assisted Selection for Leaf Blast Disease resistance and other yield traits in rice (Oryza sativa L.) using RAPD Markers

Keywords: Bulked-segregant Analysis , Single-marker Analysis , Magnaporthe grisea (Hebert) Barr , Moroberekan , Grain yield , Glume Purple Tip.

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Abstract:

A segregating population of 220 F2 individuals was developed by raising the selfed F1 seeds of a single hybrid plant, White Ponnix Moroberekan. Two hundred and twenty F3 families were phenotyped for blast resistance under artificial condition. Bulksegregantanalysis resulted in the identification of 12 leaf blast resistant phenotype specific RAPD markers. Mapping survey wasdone with seven RAPD primers which co-segregated in BSA. Twelve RAPD markers were scored in the F2 mapping population.Out of 12 marker loci, 11 marker loci (91.97 per cent) fitted into the expected segregation ratio of 3:1 based on χ2- test at 0.05per cent probability value. One way ANOVA was performed to identify the marker phenotype association and this resulted in theidentification of seven RAPD markers linked to three traits viz., leaf blast resistance (OPBB 5258, OPBB 5194, OPAL 16940 andOPBD 12680), five markers for GPT and one marker for grain yield per plant.

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