Herbal medicines have good efficacy, safety, and lesser side effects. They have great demand in developed world for primary health care. India has rich traditional knowledge, heritage of herbal medicines and large biodiversity but despite it India has dismal share of world market. Many Pharma companies marketed herbal preparation as neutraceutical and took excuses from quality control parameter set by W.H.O. India has thousands of medicinal plants but in Indian Pharmacopoeia. Quercetin, one of the most abundant natural flavonoids, presents in daily food. Quercetin is of interest because of its pharmacological function. The quantitative determination of flavonoids compound in seeds of Elaeocarpus ganitrus was carried out in high performance thin layer chromatography. Concentration of quercetin in Elaeocarpus ganitrus seeds was calculated based on calibration curve.