In underdeveloped countries, traditional and herbal medicines including folk medicinal practice scatters to nearly 70% of the population because of accessibility, affordability and the time tested dependability. They still depend on herbal medicine because of the side effect of most of the modern drugs. The medicinal activities are due to the presence of active principle in them. We have chosen the plant Dichrostachys cinerea (L) belonging to the family Mimosaceae for preliminary phyto chemical screening and to study the antimicrobial activity. Dichrostachys cinerea are traditionally used for antimicrobial, anticonvulsant. astringent (root); antihelminthic, purgative, laxative and diuretic (bark). In medicine, bark is used to alleviate headache, toothache, dysentery, elephantiasis and root infusions are consumed to treat leprosy, syphilis coughs, as an anthelmintic, purgative and strong diuretic. The leaves are particularly useful and can be taken to treat epilepsy and can also be taken as a diuretic and laxative, and its powder can be used in the massage of fractures. Based on the ethano pharmacological information’s, the present study is focused to evaluate Preliminary phytochemical and antimicrobial activity.