Pterospermum. Xylocarpum (Family: Sterculiaceae) is a wild medicinal plant distributed throughout India and south East Asia. It is used against treatment of joint diseases like gout, rheumatism, arthritis. In present study acetone, hexane, chloroform and methanol extracts were prepared from the leaf of P.xylocarpum and phytochemical analysis was done to screen alkaloids, steroids, saponins, tri terpinoidal saponins, carbohydrates, flavonoids, polyphenols, tannins and glycosides. Antimicrobial activity was tested against nine bacterial and nine fungal species. Among the four extracts methanol and acetone extracts showed significant anti bacterial activity and there is no significant activity against fungal strains and it was found to be effective against two tested bacterial species viz, xanthomonas campestis and E.Coli. Methanol crude extract was prepared by the cold maceration process and it was optimized for the yield, the crude extract was tested for anti bacterial activity at different concentrations viz, 250,500,1000mg/ml among them 500mg/ml concentration has shown significant antibacterial activity and in the methanol crude extract the phytochemical analysis showed the presence of alkaloids, steroids, phenols and cardiac glycosides.