A new easy-to-use air purification system called “A-Bios”,based on UVC radiation with the addition of specialgraphite filters to eliminate traces of ozone, to be used inthe indoor environment has been developed in the frameworkof the EC financed project “An innovative technology,based on UV radiation, to strongly reduce the microbialactivity of the air inside the store rooms of the cultural heritageconservation institutes”. The objectives of theresearch presented in this paper were to evaluate the relativeefficiency of the A-Bios air purification system in theabatement of airborne fungal spores’ vitality and to determinethe effect of time exposure to A-Bios on the fungalcolony forming units (CFU) recovery. Aerobiological monitoringwas conducted in the “David Lubin memorial librar y”of the Food and Agriculture Organization (FAO) in Romebefore and after operation of the A-Bios system. The resultsobtained in this research indicate that the differencebetween fungal airborne concentration data before andafter A-Bios treatment is statistically significant, and independentboth on the agar type used for spores collection,the sampled air volume and the sampling heights. The A-Bios device appeared to be a promising tool to lower and tomaintain low the risk of microbial contamination in closedspaces, like libraries and other exposing spaces for culturalheritage.