The present paper deals with standardization of chandanasava, known to be effective in karsya (malnutrition), sukrameha (presence of semen in urine), mutrakrcchra (painful micturation), hrdroga (heart diseases), balaksaya (astringent), agnimandya (loss of appetite). Formulation has been standardized by following modern scientific quality control procedures for the finished product. Standardization of chandanasava was achieved by organoleptic study, physic-chemical analysis, thin layer chromatography, high performance thin layer chromatography (HPTLC). The obtained values of physical and chemical parameters can be adopted to lay down new pharmacopoeial standards to be followed for analysis of chandanasava to check batch to batch variation. Quantitative evaluation of gallic acid and apigenin in chandanasava by HPTLC had shown 25.12 μg/ml and 0.4 μg/ml respectively.