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In vitro Plantlet Regeneration from Internode Explant of Native-olive (Elaeocarpus robustus roxb.)

Keywords: Elaeocarpus robustus , internode explants , plantlet , regeneration

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Abstract:

Regeneration of multiple shoots via callus induction and organogenesis were achieved in native-olive (Elaeocarpus robustus). Callus induction and shoot buds regeneration were obtained from internode explants of Elaeocarpus robustus on their sufficient medium. The best organic callus was found on modified MS (MMS1) medium supplemented with 0.5 mg L1 BA+0.5 mg L1 2,4-D. Development of adventitious shoots occurred when the calli were subcultured on MMS1 medium supplemented with BA and NAA. Maximum frequency (80%) of calli induced adventitious shoots with highest number of 14.05 ± 1.56 shoots per callus were obtained when the medium was fortified with 1.0 mg L1 BA+0.1 mg L1 NAA. Plantlets developed roots when in vitro developed microcuttings were implanted on modified MS (MMS2) medium with 0.2 mg L1 of IBA. Within six weeks of transfer, 65% rooting was achieved on this medium. Rooted shoots (plantlets) were gradually acclimatized and successfully established under natural condition with about 50% survival rate.

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