The recent global resurgence of interest in traditional systems of medicines has led to an increase in the demand for them. These medicines are effective but commercialization of the manufacture of these medicines to meet this increasing demand has resulted in a decline in their quality, primarily due to lack of adequate regulations pertaining to this sector of medicine. The need of the hour is to evolve a systematic approach and to develop well-designed methodologies for the standardization of herbal formulations. In the paper, attempt has been made to evaluate Sulaharana Yoga, a Ayurvedic formulation. One sample was procured from manufactures and subjected to macroscopic, microscopic characterization, physico-chemical screening, thin layer chromatography (TLC) studies and was compared using in-house preparation formulation. It was observed that the commercial samples matched exactly with that of in-house preparation after performing the standardization.