Phenothiazines are heterocyclic molecules containing two benzene rings linked in a tricyclic system through nitrogen and sulfur atoms.Phenothiazine derivatives having amino alkyl side chain and these are connected to the nitrogen atom of heterocyclic unit playing crucial role in medicinal chemistry.From last few decades a considerable amount of attention has been focussed on synthesis of phenothiazines derivatives and screening them for different pharmacological activities. The investigation of substituted 10H-Phenothiazines has steadily strong growth because they exhibit a wide range of applications. These moieties are widely employed as antibacterial, antiviral, anti-inflammatory, anticancer, sedatives, tranquilizers agents etc. Slight change in substitution pattern in phenothiazine nucleus causes distinguishable difference in their biological activities. In this review we are discussing about synthesis and various biological activities of newly synthesized Phenothiazine derivatives.