Pharmaceutical study appreciates the importance of formulation preparation which ultimately determines the nature and magnitude of pharmacological activity expressed. Guduchi is one among the most important medicinal plants of India and various formulations of Guduchi ghrita are mentioned in different Ayurvedic classical texts. In the present study, a simple formulation of Guduchi ghrita which is used for treatment of chronic fever wherein no other ingredient except Guduchi was selected and a comparative pharmaceutical study of Guduchi ghrita prepared from male and female plants was carried out. The preparation of Guduchi ghrita formulations were carried out by adopting standard operative procedure. The result of this study shows that ghrita prepared from male plant yielded more quantity of finished product than that of female plant.