Trikatu Churna (TC) is an ancient traditional Ayurvedic preparation prescribed for a wide range of disorders. Though TC is an age old formulation, there are very few references on its quality control and standardization. In this work, an attempt has been made to standardize TC by qualitatively evaluating the preliminary phytochemicals. Piperine content of TC was determined using HPTLC. Evaluation of safety potential of TC samples and stability evaluation by comparative study of the in house TC formulation with marketed TC formulations with respect to their piperine content is a value addition to the current work.