The aim of this research were to study growth and protein also cyanidemushroom Auricularia polytricha in medium sawdust and tapioca solid waste with urea fertilizer added. The research had done using the completely randomized design (RAL) with 2 variables. The first variable was various urea fertilizers with 4 rates of concentration (0 g; 2 g; 4 g; 8 g and 10 g). The second variable was various mixed sawdust and tapioca solid waste with 3 rates concentration i.e. medium without tapioca solid waste, mixed sawdust and tapioca solid waste 3.5: 1.5, mixed sawdust and tapioca solid waste 3.0: 1.0. Basidiocarp on mushroom wet weight, dry weight, percentage of water, protein and cyanide was measured and then data collected were analyzed using analysis of variance (ANOVA) and followed by Duncan Multiple Range Test (DMRT) with 5% of confidence level. The use of mixed medium of sawdust and tapioca solid waste of 3: 1 with increasing urea concentration have no effect increasing growth of basidiocarp wet weight, dry weight, percentage of water and protein content of basidiocarp. On the other hand, the mixed consisting of sawdust and tapioca solid waste of 3: 1 with concentrations of 4 g and 8 g of urea have resulted in the content of cyanide.