A rapid and sensitive reverse phase HPLC method is depicted for the qualitative and quantitative assay of Bupropion hydrochloride in pharmaceutical dosage form. Bupropion hydrochloride waschromatographed on a reverse phase C18 column with a mobile phase consisting of methanol:phosphate buffer (pH-6) in the ratio of 80:20% v/v.The mobile phase was pumped at a flow rate of 1ml/min. Aceclofenac was used as an internal standard and the eluents were monitored at 223 nm. The retention time of the drug was 5.7 min.With this method, linearity is observed between area under curve (AUC, expressed in mV.min) and concentration of Bupropion hydrochloride in the injected solution, in the range of 10-200 μg/ml.The method was found to be applicable for analysis of drug in tablets. The results of the analysis were validated statistically.