The phytochemical constituents of Borassus flabellifer was identified and evaluated the antibacterial activity of the acetone, benzene, chloroform, ethanol and methanol extracts. A phytoconstituent analysis of alkaloids, glycosides, terpenoids, steroids, flavonoids, tannins, phenols and saponins were performed on the various solvent extracts. Antibacterial activity was analyzed by agar well diffusion method against five pathogenic microorganisms, E. coli, S. aureus, K. pneumonia, P. aeruginosa and B. subtilis. The maximum zone of inhibition was exhibited for E.coli (14mm); P. aeruginosa (13mm) and B. subtilis (11mm)against compared to tested micro organisms. The methanolic extract of the palm root has showed consistently significant inhibitory activity on different bacterial species tested. Furthermore, identification of bioactive compounds will be analyzed.