The aim of this study was to investigate the combined effect of modified atmosphere packaging and irradiation on increase of the shelf-life and sensory acceptance of refrigerated chicken breast fillets. The combination of the mixture 80% CO2/20% N2 and the irradiation (3kGy) increased the shelf-life from 5 to 16 days. The lactic acid bacteria and Aeromonas spp. were the most resistant to radiation and high concentration of CO2, while the enterobacteria, coliforms and Listeria spp. showed the greatest sensitivity. The combined technology provides chicken breast fillets with longer shelf-life and safety to the consumer, without changing the flavor and aroma of food and it may be suggested as an alternative in the food industry.