About 6 extract (methanol, acetone, hexane, ethyl acetate, hexane-ethyl acetate, aqueous extraction) from the leaves of Mangifera indica was extracted. Hexane-ethyl acetate was characterized by Gas Chromatography- Mass Spectroscopy. Ten constituents from 10 peaks were identified. Terpinyl acetate (5.80%) and phytol isomer (5.12%) are as the major constituents and the minor constituents like oxirane (3.57%), sabinene (3.24%), beta-pinen (3.34%), beta-myrcene (3.23%) , cymene (3.68%), alpha-limonene (2.82%), eucalyptol (1,8-cineo (4.71%), 1,3-benzodioxole, 5-(2-, (3.68%) were identified. The antimicrobial activity of different extract was tested against human and plant pathogenic bacteria. Hexane-ethyl acetate extract showed significant role on inhibiting almost all tested pathogenic organisms. Using histochemical reagents such as Phloroglucinol-HCl, Dragendorff reagent was used for the localization of lignin and alkaloids respectively. Mangifera indica showed 100% larvicide activity. The addition of various concentrations of hexane-ethyl acetate extract of Mangifera indica on the L929 cell lines showed cytotoxic activity.