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INTRASPECIFIC VARIATIONS OF 16S MITOCHONDRIAL GENE SEQUENCES OF YELLOW RICE STEM BORER,scirpopbaga incertulas (LEPIDOPTERA: CRAMBIDAE) FROM WEST JAVA

Keywords: moth , haplotypes , genetic differentiations , molecular identification

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Abstract:

Yellow rice stem borer ( ) is one of the most important rice pest insectsin Asia, including Indonesia. However, there is a lack of genetic data for this importantagricultural insect. Therefore, this study was conducted to explore intraspecific differentiationof partial 16S mitochondrial gene from Bogor, Karawang, Indramayu and Cirebon(West Java, Indonesia). Here, we reported a total of 325 bp of 16S mitochondrial gene offrom the obtained samples. Among all DNA sequences, three haplotypes of 16Smitochondrial gene were observed and submitted to GenBank under Accession Number ofGU191881, GU191882, GU191883, respectively for haplotype 1, 2, and 3. The haplotype 1was found in all surveyed locations, except Bogor. Haplotype 2 and 3 werefound only in from Cirebon and Bogor samples. These haplotype variations can be applied asDNA markers for early larva detection method among other rice stem borers.Hence, further explorations of the mitochondrial variations of in Java and otherparts of Indonesia are neededmoth, haplotypes, genetic differentiations, molecular identification1* 1 2(1(2Department of Biology, Bogor Agricultural University (IPB), Darmaga,Bogor 16680, INDONESIAIndonesian Centre for Agricultural Biotechnology and Genetic ResourcesResearch and Development (ICABGRD)Scirpophaga incertulasS. incertulasS.incertulasS. incertulasS. incertulasS. incertulasABSTRACTINTRODUCTION

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