ABSTRACT: The different fractions of crude methanol extract of stem of Stephania japonica (Thunb.) Miers (Menispermaceae) was screened for its analgesic and antidiarrhoeal activities. The different fractions of crude extract produced significant writhing inhibition in acetic acid induced writhing in mice at the dose of 250 and 500 mg/kg body weight comparable to the standard drug diclofenac sodium at the dose of 25 mg/kg of body weight. When tested for its antidiarrhoeal effects on castor oil induced diarrhea in mice, it increased mean latent period and decreased the frequency of defecation significantly at the dose of 250 and 500 mg/kg body weight comparable to the standard drug loperamide at the dose of 50 mg/kg of body weight. The overall results suggest the analgesic and anti diarrhoeal activities of the different fractions of crude extract. Among all the fractions Ethyl acetate soluble fraction showed most significant analgesic activity and Petroleum ether soluble fraction showed the most significant anti diarrhoeal activity.