Most of the traditional systems of medicine are effective but they lack of standardization. So there is need to develop standardization technique. Central Council for Research in Ayurveda has given preliminary guidelines for standardization the conventionally used formulations. Standardization of herbal formulations is essential in order to assess the quality of drugs, based on the concentration of their active principles, physical and chemical standards. This article reports on standardization of Yakrita Plihantak Churan a polyherbal Ayurvedic formulation used as hepatoprotective. Samples were collected from local market and were subjected standardized on the basis of organoleptic properties, physical characteristics, and physic-chemical evaluation. Microscopic characterization was compared with authentic ingredients as a reference. It was observed that commercial sample from market matched exactly with that of authentic standards after performing the standardization. The set parameters were found to be sufficient to evaluate the studied churn can be used as reference standard for the quality control/assurance purpose.