ABSTRACT: An UV spectrophotometric method for the quantitative determination of Temozolomide (TMZ) in bulk and capsule was developed in present work. The parameters linearity, precision, accuracy, limit of detection and limit of quantitation were studied according to International Conference on Harmonization guidelines. UV spectroscopic determination was carried out at an absorption maximum of 328 nm using 0.1N Hydrochloric acid as solvent. In the UV spectroscopic method linearity over the concentration range of TMZ was found to be 2-18 μg/ml with a correlation coefficient 0.999. The limit of detection and limit of quantification were found to be 0.5271 and 1.6454 mg/ml respectively. Results of the analysis were validated statistically and by recovery studies. The proposed method is simple, rapid, precise, accurate and reliable and can be used for the routine quantitative analysis of TMZ in bulk and pharmaceutical formulation.