ABSTRACT: The herbal preparation of the leaves of Solanum pubescens Pers (Solanaceae) is used in traditional medicine for the treatment of Epilepsy and febrile convulsions. A pilot study has confirmed the potency of the leaf of Solanum pubescens in the control of seizure in mice. The current study was aimed to identify the active phytochemical responsible for the activity. Results indicated that methanolic extract of Solanum pubescens at 300 mg/kg exhibited a significant P<0.05 delay in the latency of myoclonic spasms and tonic-clonic phase of seizure induced by PTZ, and also decreased the latency and increased the duration of phenobarbitone induced sleeping time. While the phytochemical studies showed the presence of alkaloids, resins, glycosides, carbohydrates, reducing sugar, fats and oils, flavonoids and terpenoids. The results suggested that the extract possessed anticonvulsant activity and central depressant effects which may be attributable to the flavonoids.