The purpose of this research was to examine penetration peculiarities of rapeseed oil injected into the combustion chamber of a diesel engine. For conducting tests, a stand imitating conditions (air density) for the engine combustion chamber was designed. The analysis of pictures obtained using a fast recording camera determined fuel injection into the chamber volume and calculated the velocity of spray head. It was established that fuel spray of injected rapeseed oil proceeds deeper into the combustion chamber than that of mineral diesel fuel. The parameters of fuel spray are mainly influenced by injection pressure rather than by the density of compressed gases.