The Hepatitis B virus (HBV) is one of the DNA Hepadnaviridae family. The mechanisms of transmission are sexual, perinatal and parenteral ones. The subjects suffering from the infection at an early age remain asymptomatic during the acute phase; but they have an increased risk of becoming chronic carriers of the disease. The objective of this study was determine the prevalence of seropositivity for Hepatitis B of donors attending voluntarily the blood bank at the Hospital “Dr. Egidio Montesinos” from El Tocuyo, discriminated against by age, gender and origin. A sample of 9344 serums were studied to detect antibodies against the surface antigen (antiHBsAg) and core antigen (antiHBcAg) of HBV. They were analyzed in the laboratory of the aforementioned Hospital, through an inmunoassay method of microparticles of third generation. The percentage prevalence for serumpositive cases was calculated and was stratified by age, sex and origin of the positive donor. The most common causes for rejection or deferral as a donor were also calculated. A seroprevalence of 7,87 was obtained. The rate by gender 10/9 prevailing the male. The rate for seropositive men was 9.8 greater than women and an age group with higher seropositivity for the 36 to 40 age group. Conclusion: the results of this study suggest a high prevalence of Hepatitis B among donors who attended to this service, which is catalogued as high or risk. This situation is atypical for the region which equated it to other endemic areas of the country.