Objective: To study the characteristics of eye signs in chronic hepatitis B patients with blood stasis syndrome and the association between blood stasis syndrome and eye signs. Methods: A total of 126 patients with chronic hepatitis B were randomly divided into four groups: non-blood stasis group, and mild, moderate and severe blood stasis groups. The changes of eye signs in the four groups were tested and compared. Results: There were significant differences in scores of blood stasis and eye signs among the four groups (P＜0.05 or P＜0.01). The changes of eye signs in chronic hepatitis B patients with blood stasis syndrome were different with those with non-blood stasis syndrome. There were no significant differences in each item of the eye signs among the four groups (P＞0.05).Conclusion: The eye signs of blood stasis syndrome are applicable, objective and convenient for diagnosing blood stasis syndrome in patients with chronic hepatitis B.