Background: Multiple sclerosis (MS) is one of the most frequent and debilitating disease of the nervous system. Some recent studies show the possible role for iron and ferritin in the course of MS. The aim of this study was to evaluate the level of serum iron and ferritin in patients with MS and comparision of them with control group. Materials and Methods: In this descriptive study, serum iron and ferritin were determined in 30 patients with MS and compared with 30 healthy persons, which were matched in terms of age and gender. Data were analyzed with statistical descriptive methods and and t-test.Results: In this study, 30 patients were evaluated. The mean age of both groups was 35.2. Mean serum iron in patients group was 85.16±38.38, and in control group was 91.73±27.89. Mean serum ferritin in patients group was 132.20±80.71 and in control group was 147.40±75.02. There was no significant relationship between serum iron and ferritin in both groups, and also there was no relationship between serum iron and ferritin with age and sex and the type of disease.Conclusion: This study did not show any difference between serum iron and ferritin in patients with multiple sclerosis and control group.