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Involvement of TIP60 acetyltransferase in intracellular Salmonella replication

DOI: 10.1186/1471-2180-10-228

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In an attempt to identify host proteins that interact with SseF, we conduct a yeast two-hybrid screening of human cell cDNA library using SseF as the bait. We identified that TIP60, an acetyltransferase, interacts with SseF. We showed that the TIP60 acetylation activity was increased in the presence of SseF, and TIP60 was upregulated upon Salmonella infection. In addition, TIP60 is required for efficient intracellular Salmonella replication in macrophages.Taken together, our data suggest that Salmonella may use SseF to exploit the host TIP60 acetyltransferase activity to promote efficient Salmonella replication inside host cells.Protein acetylation adds the acetyl group on either the amino-terminal residues or on the epsilon-amino group of lysine residues. Lysine acetylation affects many protein functions, including DNA binding, protein-protein interactions, and protein stability. TIP60 catalyzes histone acetylation [1,2]. It was originally identified as a cellular acetyltransferase protein that interacts with HIV-1 Tat [3]. Over-expression of TIP60 increased Tat transactivation of the HIV-1 promoter [3]. Recent studies found that TIP60 has diverse functions involved in transcription, cellular signaling, DNA damage repair, cell cycle checkpoint control and apoptosis [2,4,5].Salmonella enterica serovar Typhimurium (S. typhimurium) causes gastrointestinal diseases in humans and typhoid-like fever in the mouse. S. typhimurium encodes two Type III secretion systems within the Salmonella pathogenicity islands 1 and 2 (SPI-1 and SPI-2) that are required for Salmonella entry and subsequent survival inside the host cells, respectively [6-10]. Following entry into the host cells, S. typhimurium replicates within a membrane-bound compartment termed Salmonella-containing vacuole (SCV). Previous studies have shown that SifA, SseF and SseG are involved in the formation of Salmonella induced filaments (Sifs) that are required for maintaining the SCV [11-13].SseF, working together


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