Berberis aristata is an important medicinal plant of family Berberidaceae. It is commonly known as Zarishk and Daruhaldi. It is mainly used for the treatment piles, liver diseases and diabetes. As the herb is used widely in the Indian traditional systems of medicine, it was thought worthwhile to develop the quality standards for its stem bark. The results of Pharmacognostic standardization of stem bark of B. aristata are very helpful in determination of quality and purity of the crude drug and its marketed formulation.