To study the in vitro antibacterial activities of mucoadhesive suspensions containing Ciprofloxacin, three different formulations were prepared by using three polymers, such as Hydroxypropyl methylcellulose (HPMC) (S1), Carbapol934 (S2) and Carbapol940 (S3), along with some common ingredients (bases). For the investigation, agar well diffusion method was performed taking Staphylococcus aureus (ATCC 25923), Bacillus subtilis and Escherichia coli (ATCC 25922). Apart from S. aureus, S1 and Ciprofloxacin in distilled water (S4) produced more or less similar zones of inhibition against all the strains used. S2 was more potent than S4 against S. aureus, while S3 was not more effective than S4 as far as their antibacterial activities were concerned. Moreover, S4 was not inferior to Ciprofloxacin in citrate buffer, marketed product containing Ciprofloxacin and Ciprofloxacin disc. The negative controls of the study, i.e., the different bases, distilled water and citrate buffer did not show any antibacterial activity. Considering the overall antibacterial activity pattern of different formulations, it may be concluded that S1 was the most potent, while S2 was more effective than S3. In addition, S1 and S4 were almost equally potent against all the strains.