Typhoid fever is a prolonged and debilitating fever which is of major public health concern in many countries.Laboratory diagnosis of typhoid fever requires isolation and identification of Salmonella enterica serotype Typhi. In many areas where this disease is endemssssic, laboratory capability is limited. Recent advances in molecular immunology have led to the identification of sensitive and specific markers for typhoid fever and technology to manufacture practical and inexpensive kits for their rapid detection. Three commercial kits for serologic diagnosis of typhoid fever. Patients presenting with 4 days of fever were enrolled at two hospitals. Patients with serotype Typhi isolated from blood samples, and controls were patients with other laboratory-confirmed illnesses. This paper described following methods for diagnosis in case of typhoid fever, Laboratory analysis. (i) Blood culture (ii) Confirmation and antimicrobial susceptibility testing of isolates (iii) Investigation of stool culture (iv) Widal test. (v) Rapid tests (vi) A polymerase chain reaction (PCR)-based test.