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Polymorphisms of calpastatin gene in sheep Polimorfizm genu kalpastatyny owiec

DOI: 10.5513/jcea01/12.3.934

Keywords: polimorfizm , kalpastatyna , owce , pcr -rflp

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Abstract:

Calpastatin plays an essential role in the growth of skeletal muscles and post mortem meat tenderness. The objective of this research was to determine polymorphism in the calpastatin gene in a group of 212 sheep (192 ewes and 20 rams) of four breeds: Polish Merino, Berrichon du Cher, Blackheaded Mutton Sheep, and Ile de France. Polymorphism was identified using the PCR -RFLP technique in accordance with methods by Palmer et al. [9]. The amplified product with the length of 622 bp was digested with restriction enzymes MspI and NcoI. It was found that the M and N alleles were present in CA ST/MspI locus, their frequency being 83.5% and 16.5% respectively. Whereas in CA ST/NcoI locus the M allele occurred with the frequency of 95.8%, and the N allele with the frequency of 4.2%. Kalpastatyna odgrywa wa n rol we wzro cie mi ni szkieletowych i procesie kruszenia mi sa post mortem. Celem badań by o okre lenie polimorfizmu w genie kalpastatyny w grupie 212 owiec (192 maciorki i 20 tryków) czterech ras: merynos polski, berrichon du cher, czarnog ówka i ile de france. Polimorfizm zosta zidentyfikowany metod PCR -RFLP wed ug metodyki Palmera i wsp. [9]. Zamplifikowany produkt o d ugo ci 622 pz poddano trawieniu enzymami restrykcyjnymi MspI i NcoI. Badania wykaza y wyst powanie alleli M i N w locus CA ST/MspI z frekwencj odpowiednio 83,5% i 16,5%. Natomiast w locus CA ST/NcoI allel M wyst pi z cz sto ci 95,8%, a allel N z cz sto ci 4,2%.

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