Oxidative damage is involved in the pathogenesis of various hepatic injuries. In the present study, the protectivity of methanolic extract of leaves of Adiantum incisum forsk (MEAI), as an antioxidant to protect against CCl4-induced oxidative stress and hepatotoxicity in Albino Wistar rats was investigated. Intraperitoneal injection of CCl4, produced a marked elevation in the serum levels of serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP) and total bilirubin, and decrease in the total protein. Histopathological analysis of the liver of CCl4-induced rats revealed marked liver cell necrosis with inflammatory collections that were conformed to increase in the levels of SOD, GPx, LPO and CAT. Daily oral administration of methanolic extract of A.incisum forsk at 100 and 200 mg/kg doses for 10 days produced a dose-dependent reduction in the serum levels of liver enzymes. Treatment with A.incisum forsk normalized various biochemical parameters of oxidative stress and was compared with standard Silymarin. Therefore, the results of this study show that A.incisum forsk can be proposed to protect the liver against CCl4-induced liver damage in rats, and the hepatoprotective effect might be correlated with its antioxidant and free radical scavenger effects.