The research was based on the 2001-03 field experiment set up on Luvisol, of a very good rye soil complex, IIIb soil valuation class. The soil was slightly acidic in reaction, average richness in available forms of phosphorus, potassium and magnesium as well as low richness in the content of sulphates (VI). The research demonstrated that the sulphur fertilisation applied showed, in general, a slight effect on the content and equivalent proportions of macroelements in oat grain. The fertilizers (ammonium sulphate (VI), potassium sulphate (VI) and Wigor S) applied, irrespective of the sulphur form and dose, in some of the research years there was shown a slight decrease as compared with the non-fertilisation treatment, the contents of phosphorus, potassium and calcium. The content of magnesium, especially sulphate sulphur (VI) in oat grain, as affected by fertilizers containing sulphur, in general, gets increased. The elementary sulphur form found in Wigor S made it possible to reach slightly higher contents of phosphorus, calcium and magnesium than its sulphate (VI) form than the one used in a form of K2SO4 and (NH4)2SO4 however, in general, these differences were not significant. Neither were there identified any differences between the effect of the 20 and 40 kg Sha-1 doses on the content of the macroelements.