Genetic diversity and genetic differentiation among Polish populations of Norway spruce (Picea abies (L.) Karst.) were studied analyzing allozyme variation at 24 loci. Presented investigations based on 81 clones represented 6 populations from three different regions: Sudety Mts., Beskid Mts. and North-East Poland. On average, the expected heterozygosity was 0.134, 52.8% of loci were polymorphic, and the number of alleles per locus was 1.8. Our data confirm the hypothesis that a lower level of variation of Picea abies exists in central Europe, compared to other regions of its natural distribution. Relatively small allozyme differentiation among regions was observed (Fst =0.027, and Nei's genetic distances ranged from 0.005 to 0.008). Reasons for the low genetic differentiation observed between Southern and Northern populations are briefly discussed.