Aims: Urogenital tract infection (UTI) contributes to the commonest single defined cause of infertility worldwide. To evaluate the role of urogenital tract infection in male with infertility and its association with sperm quality. Methodology and Results: Three hundred and twenty three (323) samples from infertile male subject were screened microbiologically for microorganisms associated with urogenital tract infection with seventy-two (72) age-matched male as controls using microbiological standard procedure. 164 (50.8%) infection rate was recorded. The dorminant uropathogen detected or isolated were Staphylococcus aureus (14.0%), Chlamydia trachomatis (11.4%), Escherichia coli (4.3%), Micoplasma genitalium (4.0%) Klebsielli aerogenes (4.0%). Others were Staphylococus saprophyticus, Pseudomonas aeruginosa, Protein mirabilis with 2.7% each respectively, Protein vulgaria treponema pallidum (2.1%), Schistosoma haematobium (0.9%) Wulchereria Bancrofti (0.3%), Human immune virus (2.7%). Semen profile of the male patients with urogenital tract infection had abnormal semen quality in this study P<0.05. Conclusion, significance and impact of study: Oligospermic infertile male subjects should be screened for urogenital tract infection to further enhance good quality sperms and functions.