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Genetic Diversity of Pandanus from West Java based on ISSR Markers

Keywords: genetic diversity , ISSR , Pandanus , West Java

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Abstract:

Genetic Diversity of Pandanus from West Java based on ISSR Markers. The genetic diversity of 10 species ofPandanus from West Java was examined using inter simple sequence repeat (ISSR) technique. Samples were collectedfrom different localities in West Java. Two primers (ISSR2 and ISSR7) were selected as reliable amplifying ISSRmarkers. The two primers amplified 19 band position, with amplification size ranged from 200 to 1500 bp. Geneticdistance for samples ranged from 0.267 to 0.957. Genetic distance was high (0.957) between P. pseudolais and P.spinistigmaticus, and between P. kurzii and P. pseudolais, where as genetic distance was low (0.267) between P.scabrifolius and P. bidur.

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