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Study of the effects of breed on some innate immunity parameters in rams

Keywords: sheep , indigenous breeds , phagocytic activity , NBT test , total plasma protein

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Abstract:

Investigations were carried out on 26 rams from the breeds Karakachan and Copper-Red Shoumen. The non-specific immune parameters, phagocytic activity of leukocytes, bactericidal activity of phagocytes systems (oxygen-dependent and oxygen independent) and total plasma protein level were evaluated. Phagocytic response was evaluated against S. aureus 209-P with a certain percentage of active phagocytes (phagocytic index) and the number of absorbed particles per one phagocytic cells (phagocyte number). Phagocytosis completion index was defined as the percentage of the microbial cells that have been destroyed by phagocytes after incubation. State of the oxygen-dependent bactericidal systems of phagocytes was assessed in vitro using the NBT test, which reflects the ability of superoxide restore NBT in diphormazane. NBT test was evaluated by the degree of reduction in spontaneous and stimulated reactions, taking into account the intracellular deposits diphormazane. Our studies and results shows that the rams from the two local Bulgarian breeds have a high activity of innate immune parameters and that’s may be useful and important in the breeding programs as an indicator of resistance and highly tolerance to oxidative stress.

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