The effect of a single strain probiotic Enterococcus faecium DSM 7134, upon some biochemical and haematological parameters in broilers, was examined after a single dose application of ochratoxin A (OTA). For that purpose, a total of 160 Ross broiler chicks were randomly distributed into four different treatments with two replicates of 20 chicks each: 1) control; 2) probiotic 3.300 x 109 CFU, tested at 0.2 g/l in drinking water; 3) OTA 50 mg/l, in drinking water; and 4) probiotic plus OTA, in same amounts as in groups 2 and 3. Blood samples were collected by cardiac puncture 6 h and 12 h after ochratoxin A application. Evaluated parameters in chicks’ blood were: total protein, albumin, urea, creatinine, haematocrite and haemoglobin. Data for all parameters at 12 hours after OTA application, revealed significant (p<0.01) differences in group receiving only mycotoxin (OTA) when compared with data from the chicks in group receiving probiotic and ochratoxin (P+OTA). Hematological analyses showed signifi cant (p<0.01) decreased hemoglobin value in chicks from group OTA at 12 hours post exposure to mycotoxin, compared to those at 6 hours post exposure. Chicks from the group receiving only probiotic, at 12 hours showed significant (p<0.01) decrease in creatinine value compared to that of the control group. It can be concluded, that administration of probiotic Enterococcus Faecium DSM 7134 in drinking water together with ochratoxin, may well be a contribution to a healthy broiler production, especially if probiotic is used as an integral part of a larger concept including management factors.