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PHYTOCEUTICAL EVALUATION AND ANTIMICROBIAL PROPERTIES OF Eclipta alba AND Tylophora indica

Keywords: Phytochemical , antibacterial activity , inhibition zone , Eclipta alba , Tylophora indica alcoholic and aqueous extracts

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Abstract:

Since plants are used as therapeutic agents, the present study was conducted to evaluate the phytochemical profile and antibacterial activities of extracts of. Eclipta alba and Tylophora indica .Studies on the antibacterial activity of methanol, petroleum ether and aqueous extracts of dry leaf, stem and root of Eclipta alba and Tylophora indica was evaluated using zone of inhibition studies and minimum inhibitory concentration. The microorganisms used include Escherichia coli, Micrococcus roseus and Pseudomonas flavescens . The extract exhibited antibacterial activity against these experimental strains. Methanol extracts shows higher degree of antibacterial activity than aqueous and petroleum ether extracts. The minimum inhibitory concentration ranged between 12.5mg/mL and 75mg/mL depending on microorganism and various extract. Screening of the phytochemical extracts revealed the presence of compounds like alkaloids, glycosides, tannins and flavonoids. Escherichia coli and Pseudomonas flavescens are more sensitive where as Micrococcus roseus is less sensitive to extracts of Eclipta alba and Tylophora indica. The experimental observation shows that Eclipta alba and Tylophora indica have antibacterial activity and can be used for therapeutic purposes.

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