Medicinal plants have been used for many years in daily life to treat diseases all over the world. Plants considered not only as dietary supplements of living organisms but also traditionally used for treating many health problems. Medicines such as Quinine a popular antimalarial drug are obtained from the tree barks. The intention of the present study was to check the Antibacterial and Antifungal activity of barks of trees Hymenodictyon obovatum Wall. and Heterophragma quadriloculare (Roxb.) K.Schum. collected from Kumta and Dharwad region. Acetone, Alcohol and Aqueous extracts of these plants were obtained by Soxhlet method. All these extracts Acetone, Alcohol and Aqueous were tested for the inhibition activity against the different Bacterial strains Viz. staphylococcus aureus, klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus vulgaris and Fungi Candida albicans and Aspergillus niger in three different concentrations of 25mg/ml, 50mg.ml, and 100mg/ml. Hymenodictyon obovatum Wall. From Dharwad region showed the good activity against all the bacteria and the moderate activity against the fungus Candida albicans compared to the same from Kumta region. The acetone extract 100 mg/ml of Hymenodictyon obovatum Wall. From Dharwad showed inhibition zone of 24mm against Pseudomonas aureginosa which is more than the control assuring a good anti activity against Bacterium Pseudomonas aureginosa where as Heterophragma quadriloculare (Roxb.) K.Schum from both the regions didn’t showed any appreciable activity against any of the Bacteria and fungi used.