Clone selection in viticulture represents a way of eliminating the negative influence of mutation changes in the future vineyard, as well as preventing the virus-infected plants and plants infected with virus-related diseases from further propagation. Riesling Italico variety is an old variety. It has been grown in Vojvodina for centuries. The clone selection of Riesling Italico variety in Sremski Karlovci was started in 1975. In January 1991 three clones were recognized. Shortly after, the subclone selection of these three clones was initiated. This paper presents the resultants of subclone selection of Riesling Italico. Subclones SK 54-4 and SK 54-10 are characterized by good quality and the grapes of these subclones can be used for the production of high quality wines in most of the years.