Due to the low biological value of proteins of common maize, it was reinitiated breeding for high protein quality maize (HQPM) using three genetic systems, namely: opaque-2 gene, endosperm modifier genes and enhancer genes, which are increasing lysine and tryptophan content in opaque-2 background In order to alleviate effect of abiotic and biotic stress factors, the genotypes with tolerance to those factors were included. Genetic resources originating from North, Central and South America, then West, Central and Southern Africa and gene bank of Maize Research Institute “Zemun Polje” were used. Combining breeding approaches in selection of genetic resources, field plot techniques and laboratory analysis, it was created large number of early QPM varieties, inbred lines and hybrids with modified endosperm and high yield potential under poor and good growing conditions. Created lines exhibited high combining ability in conventional and non-conventional hybrids. Yield trials showed that QPM hybrids are competing with commercial hybrids of common maize.