A total of 28 species belonging to 16 genera of fungi parasitic on root-knot nematodes were isolated and identified. Aspergillus terreus, A. nidulans, A. tamarri and Fusarium anthophellum are appeared to be new report on root-knot nematode and not reported hitherto. Of the fungi isolated, Aspergillus flavus, A. fumigatus, A. niger, Aspergillus sp., Acremonium butyri, Alternaria alternata, Catenaria sp. Cephalosporium sp., Cladosporium cladosporoides, Cladosporium sp., Cunninghamella elegance, Curvularia lunata, Fusarium exosporium, Fusarium sp., Ulocladium atrum and a sterile fungus are new records on root-knot nematodes in Pakistan. Natural infestation of Paecilomyces lilacinus and Arthrobotrys sp. is also recorded first time from Pakistan.