The present study designed to investigate the antibacterial activity of honey obtained from different state of India. A total of 10 honey samples (five from Uttaranchal state and five from Uttar Pradesh state) were investigated for their antibacterial activity against antibiotic resistant bacterial isolates of S. epidermidis and E. coli using the disc diffusion method. Marked variations were observed in the antibacterial activity of different sample of honey. Three (60%) of the five Uttaranchal samples and two (40%) of the five Uttar Pradesh samples showed excellent antibacterial activity against gram-positive and gram-negative bacteria. Both Uttaranchal state and Uttar Pradesh state honey samples possess in vitro antibacterial activity against antibiotic resistant isolates of S. epidermidis and E. coli bacteria at 400 uL/disc quantity of 60% concentration (v/v).