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Detection of Campylobacter jejuni in raw meat

Keywords: Campylobacter jejuni , foodborne infection , raw meats

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Abstract:

Background & Objectives: Campylobacter jejuni is a Gram negative, microaerophilic, non-spore-forming and a small"ncurved bacillus which is able to cause foodborne infection in human. In this study the occurrence of C. jejuni in poultry and"nbeef meat was investigated."nMaterials & Methods: Forty raw meat samples including 22 poultry samples and 18 beef samples were investigated for the"npresence of C. jejuni. To isolate the bacterium, the samples were initially enriched in Preston Broth medium and subsequently"ntransferred to Campylobacter selective Agar containing defibrinated sheep blood and antibiotics. The biochemical tests were"nused for identification of isolated bacteria at species level."nResults: Three poultry samples were positive for C. jejuni."nConclusion: Alimentary tract of chickens contain high numbers of C. jejuni therefore, this bacterium can be easily found in"ntheir feces. It is recommended to use chlorinated water in birds’ feed and to perform slaughtering, skinning and evisceration"nunder aseptic conditions to prevent campylobacteriosis in human from poultry meat. None of the beef samples yielded any"nCampylobacter, this may be due to limited number of samples of beef meat analyzed in this study.

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