The use of Vegetative Compatibility Group (VCG) analysis in this study was to identify the isolates and the distribution of Fusarium oxysporum f. sp. cubense (Foc) as well as the infected banana varieties. Samples of Foc isolates were collected from the diseased banana plants in Lampung province. While isolating and purifying the isolates with single spore techniques and analyzing the VCG were performed in the laboratory. Fifteen testers of VCGs used were from Australia. The results revealed that 30 isolates of the pathogen Foc have been found at all surveyed locations on 10 banana varieties. These varieties were seven varieties of dessert bananas i.e., Seribu (AA), Muli (AA), Ambon Kuning, Ambon Hijau and Cavendish (AAA), Raja Sere and Raja Bulu (AAB) and three varieties of cooking bananas i.e., Nangka (AAB), Jantan (AAB) and Kepok (ABB/BBB). Seventeen of these 30 isolates were VCGs 01213/16 (TR4) found in seven banana varieties. The other isolates comprised five isolates of VCGs 0120/15 (subTR4) in three banana varieties, three isolates of VCGs 01216 (TR4) in two varieties, one isolate of VCG 01213 (TR4) in one variety and four isolates of unknown VCGs in three varieties. Since VCGs 01213/16 (TR4) was the most prevalent, managing and preventing further spread of the disease in Lampung province should be taken into account.