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A novel method of DNA shuffling without PCR process

Keywords: DNA shuffling,restriction enzyme,tissue plasminogen activator
DNA滑移
,限制酶,组织纤溶酶原,并行序列,试管内进化,识别位,变异

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Abstract:

Most DNA shuffling methods currently used require PCR process. A novel method of DNA shuffling without PCR process is described, taking advantage of the feature of some restriction enzymes whose recognition sites differ from their cleavage sites, thus giving rise to different cohesive ends.These cohesive ends can be rejoined at their native sites from different parental sequences, generating new sequences with various combinations of mutations.

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